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Creatinine amidohydrolase (creatininase; EC 3.5.2.10) from Pseudomonas putida has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. The crystal belongs to the orthorhombic space group P212121, with unit-cell parameters a = 102.0, b = 150.7, c = 167.1 Å. Native data were collected to 1.8 Å resolution by a rotation method at 100 K using an ADSC Quantum 4R CCD detector with synchrotron radiation.

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