Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 63, Part 5 (May 2007)

structural genomics communications

Acta Cryst. (2007). F63, 369-374 [ doi:10.1107/S1744309107018945 ]

Structure of O67745_AQUAE, a hypothetical protein from Aquifex aeolicus

V. Oganesyan, P. D. Adams, J. Jancarik, R. Kim and S.-H. Kim

Abstract: Using single-wavelength anomalous dispersion data obtained from a gold-derivatized crystal, the X-ray crystal structure of the protein 067745_AQUAE from the prokaryotic organism Aquifex aeolicus has been determined to a resolution of 2.0 Å. Amino-acid residues 1-371 of the 44 kDa protein were identified by Pfam as an HD domain and a member of the metal-dependent phosphohydrolase superfamily (accession No. PF01966). Although three families from this large and diverse group of enzymatic proteins are represented in the PDB, the structure of 067745_AQUAE reveals a unique fold that is unlike the others and that is likely to represent a new subfamily, further organizing the families and characterizing the proteins. Data are presented that provide the first insights into the structural organization of the proteins within this clan and a distal alternative GDP-binding domain outside the metal-binding active site is proposed.

PDB reference: 2hek

Keywords: 067745_AQUAE; Aquifex aeolicus; HD domains.