research papers
The X-ray fluorescence data from X-ray microprobe and nanoprobe measurements must be fitted to obtain reliable elemental maps. The most common approach in many fitting programs is to initially remove a per-pixel baseline. Using X-ray fluorescence data of yeast and glial cells, it is shown that per-pixel baselines can result in significant, systematic errors in quantitation and that significantly improved data can be obtained by calculating an average blank spectrum and subtracting this from each pixel.
Keywords: X-ray fluorescence; per-pixel baselines; X-ray microprobes; elemental maps; X-ray nanoprobes.
Supporting information
Portable Document Format (PDF) file https://doi.org/10.1107/S1600577519000651/rv5095sup1.pdf |