Buy article online - an online subscription or single-article purchase is required to access this article.
Download citation
Download citation
link to html
Macromolecular crystallization remains a bottleneck in structure determination by X-ray diffraction. Based on the data reflecting success rates of crystallization conditions in different screens and the information derived from the BMCD and other related studies, a simplified screen has been designed to increase the success rate of traditional screening and to save samples, time and cost. The screen has been tested with six protein samples which had been crystallized before and its comparison with Crystal Screen (Hampton Research) was also performed with lysozyme crystallization. The experimental results show that for obtaining crystal leads, the success rate of the simplified screen is reasonably higher. In addition, it has been successful in crystallizing two new proteins from snake venom using the simplified screen that had failed with Crystal Screen. These results indicate that the simplified screen, which assembles and optimizes the efficient crystallization conditions from distinct screens, extends the region of crystallization and improves the success rate of screening. Based on information of newly published efficient crystallization conditions, the simplified screen could be developed and optimized continuously in the future.

Subscribe to Acta Crystallographica Section D: Biological Crystallography

The full text of this article is available to subscribers to the journal.

If you have already registered and are using a computer listed in your registration details, please email support@iucr.org for assistance.

Buy online

You may purchase this article in PDF and/or HTML formats. For purchasers in the European Community who do not have a VAT number, VAT will be added at the local rate. Payments to the IUCr are handled by WorldPay, who will accept payment by credit card in several currencies. To purchase the article, please complete the form below (fields marked * are required), and then click on `Continue'.
E-mail address* 
Repeat e-mail address* 
(for error checking) 

Format*   PDF (US $40)
   HTML (US $40)
   PDF+HTML (US $50)
In order for VAT to be shown for your country javascript needs to be enabled.

VAT number 
(non-UK EC countries only) 
Country* 
 

Terms and conditions of use
Contact us

Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds