Structure of the HECT domain of human WWP2

WWP2 is a HECT-domain ubiquitin ligase of the Nedd4 family, which is involved in various important biological processes, such as protein degradation, membrane-protein sorting and transportation, the immune response, pluri­potency of embryonic stem cells, tumourigenesis and metastasis. The HECT domain provides the intrinsic ubiquitin ligase activity of WWP2. Here, the expression, purification, crystallization and crystallographic analysis of the HECT domain of human WWP2 (HECT^WWP2) are reported. HECT^WWP2 has been crystallized and the crystals diffracted to 2.50 Å resolution. They belonged to space group P4₁2₁2 and the structure has been solved via molecular replacement. The overall structure of HECT^WWP2 has an inverted T-shape. This structure displays a high degree of conservation with previously published structures of Nedd4 subfamily members.