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Changes in d-spacing have been measured for organic compounds as a function of temperature, wavelength and primary beam intensity using intense synchrotron radiation. The d-spacings of organic and protein crystals were found to increase irreversibly as a function of radiation dose. The activation energy of this thermally activated process was estimated. No evidence for a significant temperature increase of the sample due to exposure to intense X-ray beams could be found experimentally for flux densities up to 4 × 1012 photons s-1 mm-2.

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