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For small-angle X-ray scattering (SAXS) in solution, absolute intensity calibration is an important step to estimate the number of electrons of the sample. In this study, the excess scattering length of 1-bromododecane dispersed in dodecane was determined using that of 1-chlorododecane in the same solvent. The deviation of the experimental values from theoretical calculations obtained using atomic factors was around 1%. It is suggested that the 1-chlorododecane-dodecane system constitutes a new calibration approach that is applicable to various materials over a large energy range generally used in SAXS measurements, and could be less complex and more reliable than conventional methods.

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