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crystallization communications
Triosephosphate isomerase from methicillin-resistant Staphylococcus aureus (MRSA252) was cloned in pQE30 vector, overexpressed in Escherichia coli M15 (pREP4) cells and purified to homogeneity. The protein was crystallized from 1.6 M trisodium citrate dihydrate pH 6.5 using the hanging-drop vapour-diffusion method. The crystals belonged to space group P43212, with unit-cell parameters a = b = 79.15, c = 174.27 Å. X-ray diffraction data were collected and processed to a maximum resolution of 1.9 Å. The presence of two molecules in the asymmetric unit gave a Matthews coefficient (VM) of 2.64 Å3 Da-1, with a solvent content of 53.63%.