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Type I restriction enzymes are multimeric proteins that consist of three subunits. The HsdS and HsdM subunits form a complex protein that shows methyl­transferase activity, while the HsdR subunit functions as an endonuclease as well as as a translocase. Of these three subunits, no structural information on the HsdR subunit is yet available. The putative HsdR gene from Vibrio vulnificus YJ016 (HsdR_Vv) was cloned and expressed and the expressed protein HsdR_Vv was purified. HsdR_Vv was crystallized from 8%(w/v) polyethylene glycol 3350, 0.15 M ammonium chloride, 0.1 M HEPES pH 7.5 and 2 mM β-­mercapto­ethanol. Diffraction data were collected to 2.60 Å resolution using synchrotron radiation. The crystal belongs to the orthorhombic space group P212121, with unit-cell parameters a = 71.01, b = 89.04, c = 113.66 Å. With one HsdR_Vv molecule in the asymmetric unit, the Matthews coefficient was 2.14 Å3 Da−1 and the solvent content was 42%.

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