Download citation
Download citation
link to html
Sitting-drop protein crystallization is not used as commonly as the hanging-drop method for crystal optimization owing to the limitations of commercially available sitting-drop bridges, particularly when they are used in conjunction with 24-well crystallization plates. The commercially available sitting-drop bridge, containing space for only a single drop, restricts their wider use. Proteins that preferentially crystallize under sitting-drop conditions therefore require more work, time and resources for their optimization. In response to these limitations, and using 3D printing, a new sitting-drop bridge has been designed and developed, where five crystallization drops can be placed simultaneously in each well of a 24-well crystallization plate. This significantly simplifies the process and increases the potential of sitting drops in crystal optimization, reducing costs and hence overcoming the limitations of current approaches.

Supporting information

pdf

Portable Document Format (PDF) file https://doi.org/10.1107/S1600576718017545/ei5036sup1.pdf
MSDS for 3D printing resin

zip

Zip compressed file https://doi.org/10.1107/S1600576718017545/ei5036sup2.zip
Microbridge design file


Follow J. Appl. Cryst.
Sign up for e-alerts
Follow J. Appl. Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds