Crystallization of the AAA domain of the ATP-­dependent protease FtsH of Escherichia coli

Krzywda, S.; Brzozowski, A.M.; Karata, K.; Ogura, T.; Wilkinson, A.J.

doi:10.1107/S0907444902006972

Crystallization of the AAA domain of the ATP-dependent protease FtsH of Escherichia coli

S. Krzywda, A. M. Brzozowski, K. Karata, T. Ogura and A. J. Wilkinson

FtsH is a membrane-anchored ATP-dependent protease that degrades misfolded or misassembled membrane proteins as well as a subset of cytoplasmic regulatory proteins. It belongs to the family of AAA⁺ ATPases with roles in diverse cellular processes. The ATPase domain of FtsH from Escherichia coli has been crystallized from ammonium sulfate solutions and crystals diffracting to 1.5 Å resolution have been obtained.

Keywords: ATP-dependent proteases; FtsH; AAA domains.

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Crystallization of the AAA domain of the ATP-­dependent protease FtsH of Escherichia coli

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Crystallization of the AAA domain of the ATP-dependent protease FtsH of Escherichia coli