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Using the high flux of a third generation synchrotron sources at ESRF and ELETTRA, structural changes of the chaperonin system GroE were analyzed by time-resolved small angle scattering. A fast mixing device were established which permits determination of the solution scattering parameters of the E.coli chaperonin system GroE in time slices of 300msec. Using this device, the r eaction pathway of the two component system, GroEL and GroES was analyzed.

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