Buy article online - an online subscription or single-article purchase is required to access this article.
research papers
The radii of gyration of 16S rRNA, 30S proteins and S4 have been measured in situ in the 30S ribosomal subunit from Escherichia coli by the three-isotope method (TIM) [Pavlov & Serdyuk (1987). J. Appl. Cryst. 20, 105-110]. The values obtained were 70.0 (25), 66.7 (24) and 23.5 (10) Å, respectively. The data for ribosomal protein and RNA in this system are consistent with earlier findings, verifying that the TIM is reliable. The estimate obtained for S4 is one of the first high-quality estimates of the radius of gyration obtained for a ribosomal protein in situ.