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The absolute optical chirality of potassium lithium sulfate (KLiSO4) at room temperature has been determined by anomalous X-ray dispersion combined with observation of the rotation of plane-polarized light. The crystal used for this study was dextrorotatory along [00.1] and was carefully checked for the possibility of twinning; it was found to be single domain to within the limit of error. Finally, it was established that this crystal has the same structural chirality as that of the crystal whose structure was published by Schulz, Zucker & Frech [Acta Cryst. (1985). B41, 21-26].