addenda and errata\(\def\hfill{\hskip 5em}\def\hfil{\hskip 3em}\def\eqno#1{\hfil {#1}}\)

Journal logoSTRUCTURAL BIOLOGY
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ISSN: 2053-230X

Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of DapA (Rv2753c) from Mycobacterium tuberculosis. Corrigendum

aEMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany
*Correspondence e-mail: msweiss@embl-hamburg.de

(Received 7 November 2007; accepted 7 November 2007; online 20 December 2007)

A correction is made to the Experimental methods section of the article by Kefala & Weiss [(2006), Acta Cryst. F62, 1116–1119].

The first sentence of the Experimental methods section of the article by Kefala & Weiss (2006[Kefala, G. & Weiss, M. S. (2006). Acta Cryst. F62, 1116-1119.]) should read as follows: Each gram of cell pellet was dissolved in 10 ml buffer A [20 mM Tris pH 8.0, 250 mM NaCl, 10 mM imidazole, 5%(v/v) glycerol, 2 mM β-mercaptoethanol] plus 10%(v/v) 10× BugBuster (Novagen) and one Complete Mini EDTA-free protease-inhibitor cocktail tablet (Roche) per 20 ml, and then lysed by sonication four times for 5 min at a time using 0.25 s pulses at 277 K.

Footnotes

Present address: Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA

Acknowledgements

The authors would like to thank Dr Simone Weyand, Imperial College London, UK, for pointing out an ommission in the original article.

References

First citationKefala, G. & Weiss, M. S. (2006). Acta Cryst. F62, 1116–1119.  Web of Science CrossRef IUCr Journals Google Scholar

© International Union of Crystallography. Prior permission is not required to reproduce short quotations, tables and figures from this article, provided the original authors and source are cited. For more information, click here.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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