Crystallization and preliminary X-ray analysis of an arabinoxylan arabinofuranohydrolase from Bacillus subtilis

Arabinoxylan arabinofuranohydrolases (AXH) are α-L-arabinofuranosidases (EC 3.2.1.55) that specifically hydrolyse the glycosidic bond between arabinofuranosyl substituents and xylopyranosyl residues from arabinoxylan, hence their name. In this study, the crystallization and preliminary X-ray analysis of the AXH from Bacillus subtilis, a glycoside hydrolase belonging to family 43, is described. Purified recombinant AXH crystallized in the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 68.7, b = 73.7, c = 106.5 Å. X-ray diffraction data were collected to a resolution of 1.55 Å.