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The contribution of a particular atom in a molecule to the total X-ray scattering can be altered by varying the wavelength in the region of the absorption edge of the atom. It is shown that only the changes in the real part of the anomalous scattering of the atom provide significant changes in a pattern from a fibre containing molecules with helical symmetry. Changes due to the imaginary component are small and Friedel differences cannot be observed, owing to the fibre disorder. The information which can be obtained is equivalent to that given by a truly isomorphous heavy-atom derivative. For the general case this is not sufficient to provide unambiguous phase information. If a twofold axis is present at right angles to the fibre axis then the amplitudes are real and the phase problem can, in favourable cases, be solved.

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