Macromolecular crystallography with synchrotron radiation: photographic data collection and polarization correction

The design and operation of a focusing camera for high-resolution macromolecular crystallography with synchrotron radiation (SR) are described. The performance of this service-oriented instrument is evaluated on the basis of five years of use. Standard procedures for data collection, data processing and data reduction have been modified to take unusual features of the SR source into account; the effect of polarization is thoroughly discussed.