laboratory notes
A new octuple cuvette has been developed to reduce the experimental time in small-angle X-ray scattering (SAXS). Since each chamber has uniform dimensions, identical scattering curves for bovine serum albumin are obtained within an average deviation of 1%. The cuvette can be used to record SAXS curves for seven sample concentrations quasi-simultaneously by interchamber subtraction, i.e. calculating the difference in scattering intensity between the sample in any of the first seven chambers and a matching buffer in the eighth chamber. This enables fast acquisition of a data set and its extrapolation to infinite dilution, and increases the throughput of SAXS experiments.
Supporting information
Portable Document Format (PDF) file https://doi.org/10.1107/S0021889811045225/aj5182sup1.pdf |