Growth and structure of α-lactalbumin nanotubes

Partial hydrolysis of α-lactalbumin by a protease from Bacillus licheniformis under appropriate conditions leads to the formation of nanotubular structures. The growth of α-lactalbumin nanotubes and their dimensions were analysed using transmission electron microscopy, static light scattering and small-angle X-ray scattering. The scattering data were fitted using a model describing the growth of the tubes and using the form factor of a hollow tube. The cylinder diameter was calculated to be 19.9 (2) nm and the cavity diameter 8.7 (7) nm. The elongation rate of the nanotubes was about 10 nm min⁻¹ under the experimental conditions.