Crystallization and preliminary crystallographic analysis of exo-α-1,5-L-arabinofuranosidase from Streptomyces avermitilis NBRC14893

Exo-α-1,5-L-arabinofuranosidase from Streptomyces avermitilis NBRC14893 (SaAraf43A) is composed of a single-chain peptide containing a catalytic domain belonging to glycosyl hydrolase family 43 and a substrate-binding domain belonging to carbohydrate-binding module family 42. The enzyme catalyzes the hydrolysis of an α-linked L-arabinofuranosyl residue from hemicelluloses. SaAraf43A was crystallized at 293 K using the sitting-drop vapour-diffusion method. The crystals belonged to space group P2₁2₁2₁ and diffracted to a resolution of 2.2 Å.