Download citation
Download citation
link to html
A microcrystalline suspension of Bacillus lentus subtilisin (Savinase) produced during industrial large-scale production was analysed by X-ray powder diffraction (XRPD) and X-ray single-crystal diffraction (MX). XRPD established that the bulk microcrystal sample representative of the entire production suspension corresponded to space group P212121, with unit-cell parameters a = 47.65, b = 62.43, c = 75.74 Å, equivalent to those for a known orthorhombic crystal form (PDB entry 1ndq). MX using synchrotron beamlines at the Diamond Light Source with beam dimensions of 20 × 20 µm was subsequently used to study the largest crystals present in the suspension, with diffraction data being collected from two single crystals (∼20 × 20 × 60 µm) to resolutions of 1.40 and 1.57 Å, respectively. Both structures also belonged to space group P212121, but were quite distinct from the dominant form identified by XRPD, with unit-cell parameters a = 53.04, b = 57.55, c = 71.37 Å and a = 52.72, b = 57.13, c = 65.86 Å, respectively, and refined to R = 10.8% and Rfree = 15.5% and to R = 14.1% and Rfree = 18.0%, respectively. They are also different from any of the forms previously reported in the PDB. A controlled crystallization experiment with a highly purified Savinase sample allowed the growth of single crystals of the form identified by XRPD; their structure was solved and refined to a resolution of 1.17 Å with an R of 9.2% and an Rfree of 11.8%. Thus, there are at least three polymorphs present in the production suspension, albeit with the 1ndq-like microcrystals predominating. It is shown how the two techniques can provide invaluable and complementary information for such a production suspension and it is proposed that XRPD provides an excellent quality-control tool for such suspensions.

Supporting information

txt

Text file https://doi.org/10.1107/S1399004714001497/tz5050sup1.txt
Raw XRPD data (2theta, intensity) collected from a crystal suspension at room temperature during 16 h.

txt

Text file https://doi.org/10.1107/S1399004714001497/tz5050sup2.txt
Raw XRPD data (2theta, intensity) collected from a crystal suspension at 100 K during 12 minutes.

pdf

Portable Document Format (PDF) file https://doi.org/10.1107/S1399004714001497/tz5050sup3.pdf
Supporting Information: XRPD of a crystal suspension at 100 K

pdf

Portable Document Format (PDF) file https://doi.org/10.1107/S1399004714001497/tz5050sup4.pdf
Crystal contact analysis - detailed information.

PDB references: Bacillus lentus subtilisin, 4cfy; 4cfz; 4cg0


Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds