Escherichia coli stress protein YciF: expression, crystallization and preliminary crystallographic analysis

The Escherichia coli stress protein YciF was overexpressed and purified by three chromatographic steps. Crystals were obtained using ammonium sulfate as a precipitant. The YciF protein crystals diffracted beyond 2.25 Å resolution using a rotating-anode X-ray source. The lattice type is rhombohedral, with unit-cell parameters a = b = 80.0, c = 131.03 Å, α = β = 90.0, γ = 120.0°. The crystal belongs to space group R32.