Acta Crystallographica Section D

Biological Crystallography

Volume 61, Part 2 (February 2005)

research papers

Acta Cryst. (2005). D61, 123-129 [ doi:10.1107/S0907444904027064 ]

On increasing protein-crystallization throughput for X-ray diffraction studies

A. K. Shah, Z.-J. Liu, P. D. Stewart, F. D. Schubot, J. P. Rose, M. G. Newton and B.-C. Wang

Abstract: Two recent developments, a novel screening/optimization strategy that considerably reduces the number of trials required to produce diffraction-size crystals and a simple modification that doubles the screening capacity of the Douglas Instruments ORYX 1-6 protein-crystallization robot, have been implemented into a structural genomics project. The new two-step screening/optimization strategy yields diffraction-quality crystals directly from the screening process, reducing the need for further optimization. The ORYX modification involves the addition of extensions to the sample- and oil-delivery arms and software modifications that allow two plates to be set up simultaneously.

Keywords: crystallization; robots; high-throughput; combination screen.

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