Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein–DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region

Three types of protein–DNA complexes, AML1/Runx-1/CBFα(Runt)–CBFβ–C/EBPβ(bZip)–DNA (CBFα-β-C/EBPβ-DNA), AML1/Runx-1/CBFα(Runt)–C/EBPβ(bZip)–DNA (CBFα-C/EBPβ-DNA) and AML1/Runx-1/CBFα(Runt)–DNA (CBFα-DNA), were crystallized. The crystals were all orthorhombic and belonged to space groups C222₁, P2₁2₁2 and P2₁2₁2₁, respectively. The resolutions of CBFα-β-C/EBPβ-DNA and CBFα-C/EBPβ-DNA crystals were both 3 Å, while that of the CBFα-DNA crystal was 2.65 Å. Complete data sets were collected for all of the native crystals, along with MAD and MIR data sets for CBFα-β-C/EBPβ-DNA. The heavy-atom site was determined using MAD data for a gold derivative of CBFα-β-C/EBPβ-DNA.