Purification, crystallization and preliminary X-ray crystallographic analysis of human CCG1-­interacting factor B

A novel human factor CIB (CCG1-interacting factor B) has been isolated using the yeast two-hybrid system. The 22 kDa CIB protein has been expressed in Escherichia coli, purified to homogeneity and crystallized in a form suitable for crystallographic studies. The protein was crystallized in the orthogonal space group P2₁2₁2₁, with unit-cell parameters a = 43.60 (2), b = 44.45 (1), c = 110.70 (5) Å and one molecule in the asymmetric unit. The crystal diffracted beyond 2.2 Å resolution using synchrotron radiation.