Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 64, Part 4 (April 2008)

crystallization communications

Acta Cryst. (2008). F64, 304-306 [ doi:10.1107/S1744309108006775 ]

Crystallization and preliminary crystallographic analysis of a plant type III polyketide synthase that produces benzalacetone

H. Morita, M. Tanio, S. Kondo, R. Kato, K. Wanibuchi, H. Noguchi, S. Sugio, I. Abe and T. Kohno

Abstract: Benzalacetone synthase (BAS) from Rheum palmatum is a plant-specific type III polyketide synthase that catalyzes the one-step decarboxylative condensation of 4-coumaroyl-CoA with malonyl-CoA to produce the diketide 4-(4-hydroxyphenyl)-but-3-en-2-one. Recombinant BAS expressed in Escherichia coli was crystallized by the sitting-drop vapour-diffusion method. The crystals belong to space group P2₁, with unit-cell parameters a = 54.6, b = 89.6, c = 81.1 Å, [alpha] = [gamma] = 90.0, [beta] = 100.5°. Diffraction data were collected to 1.8 Å resolution using synchrotron radiation at BL24XU of SPring-8.

Keywords: benzalacetone synthases; polyketide synthases; chalcone synthases.