Acta Cryst. (2008). F64, 115-118 [ doi:10.1107/S1744309108001000 ]
Abstract: Langerin, a lectin that is specific to Langerhans cells, interacts with glycoconjugates through its carbohydrate-recognition domain (CRD). This carbohydrate binding occurs by an avidity-based mechanism that is enabled by the neck domain responsible for trimerization. Langerin binds HIV through its CRD and thus plays a protective role against its propagation by the internalization of virions in Birbeck granules. Here, the overproduction, purification and crystallization of the langerin CRD is reported. Crystals obtained by the hanging-drop vapour-diffusion method allowed the collection of a complete data set to 1.5 Å resolution and belonged to the tetragonal space group P42, with unit-cell parameters a = b = 79.55, c = 90.14 Å.
Keywords: langerin; carbohydrate-recognition domains; lectins; Strep-Tag II; Birbeck granules; HIV; host-pathogen interactions.
Copyright © International Union of Crystallography
IUCr Webmaster