Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 64, Part 2 (February 2008)

crystallization communications


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Acta Cryst. (2008). F64, 119-122    [ doi:10.1107/S1744309108000924 ]

Crystallization and preliminary X-ray characterization of queD from Bacillus subtilis, an enzyme involved in queuosine biosynthesis

N. Cicmil and L. Shi

Abstract: QueD (previously named ykvK) is one of several enzymes involved in the biosynthesis of the hypermodified nucleoside queuosine. Queuosine is incorporated into tRNA at position 34 of four tRNAs: tRNAHis, tRNAAsp, tRNAAsn and tRNATyr. The crystallization and preliminary X-ray crystallographic studies of queD are described here. The recombinant protein from Bacillus subtilis was overproduced in Escherichia coli and crystallized using the hanging-drop vapor-diffusion method from 25% PEG 600, 100 mM NaCl and sodium citrate buffer pH 5.5 at 291 K. The crystals diffract to 3.6 Å resolution and belong to the cubic space group F4132, with unit-cell parameter a = 240.88 Å.

Keywords: queD; ykvK; RNA modification; PreQ1 biosynthesis; Q biosynthesis; queuosine.

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