Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 63, Part 3 (March 2007)

crystallization communications

Acta Cryst. (2007). F63, 196-199 [ doi:10.1107/S174430910700509X ]

Preparation and preliminary X-ray diffraction analysis of crystals of bacterial flagellar sigma factor ²⁸ in complex with the ²⁸-binding region of its antisigma factor, FlgM

K. Okada, H. Ichihara, H. Takahashi, N. Fujita, A. Ishihama and T. Hakoshima

Abstract: The sigma 28 kDa ( [sigma] ²⁸) factor is a transcription factor specific for the expression of bacterial flagellar and chemotaxis genes. Its antisigma factor, FlgM, binds [sigma] ²⁸ factor and inhibits its activity as a transcription factor. In this study, crystals of the complex between Escherichia coli [sigma] ²⁸ and the C-terminal [sigma] ²⁸-binding region of FlgM were obtained. The crystals belong to space group P3₁21 or P3₂21, with unit-cell parameters a = b = 106.7 (2), c = 51.74 (3) Å, containing one complex in the crystallographic asymmetric unit. An X-ray intensity data set was collected to a resolution of 2.7 Å.

Keywords: sigma factor; ²⁸; antisigma; FlgM.