Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 63, Part 3 (March 2007)

crystallization communications

Acta Cryst. (2007). F63, 187-189 [ doi:10.1107/S1744309107004575 ]

Extracellular overproduction and preliminary crystallographic analysis of a family I.3 lipase

C. Angkawidjaja, D.-J. You, H. Matsumura, Y. Koga, K. Takano and S. Kanaya

Abstract: A family I.3 lipase from Pseudomonas sp. MIS38 was secreted from Escherichia coli cells to the external medium, purified and crystallized and preliminary crystallographic studies were performed. The crystal was grown at 277 K by the hanging-drop vapour-diffusion method. Native X-ray diffraction data were collected to 1.7 Å resolution using synchrotron radiation at station BL38B1, SPring-8. The crystal belongs to space group P2₁, with unit-cell parameters a = 48.79, b = 84.06, c = 87.04 Å. Assuming the presence of one molecule per asymmetric unit, the Matthews coefficient V_M was calculated to be 2.73 Å³ Da^-1 and the solvent content was 55%.

Keywords: family I.3 lipases.