Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 63, Part 3 (March 2007)

crystallization communications


HTML versionpdf version buy article online

Acta Cryst. (2007). F63, 183-186    [ doi:10.1107/S174430910700437X ]

Cloning, expression, purification, crystallization and preliminary X-ray crystallographic analysis of initiation factor 1 from Mycobacterium tuberculosis

G. N. Hatzopoulos and J. Mueller-Dieckmann

Abstract: Initiation factor 1 (IF-1; Rv3462c) from Mycobacterium tuberculosis, a component of the 30S initiation complex, was cloned and heterologously expressed in Escherichia coli. The protein was purified by affinity and size-exclusion chromatography and crystallized. A complete data set has been collected to high resolution. The crystals belonged to space group P21212, with two molecules per asymmetric unit which are related by translational symmetry.

Keywords: initiation factor 1; Rv3462c; Mycobacterium tuberculosis.

 bibliographic record in  format
  Find reference:   Volume   Page   
  Search:     From   to      Advanced search

Copyright © International Union of Crystallography
IUCr Webmaster