Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 63, Part 3 (March 2007)

protein structure communications

Acta Cryst. (2007). F63, 150-153 [ doi:10.1107/S174430910700379X ]

A high-resolution structure of ligand-free human glutamate carboxypeptidase II

C. Barinka, J. Starkova, J. Konvalinka and J. Lubkowski

Abstract: Human glutamate carboxypeptidase II (GCPII; EC 3.4.17.21) is an established marker for prostate-cancer diagnosis as well as a candidate therapeutic target for the treatment of diverse pathologies that involve glutamatergic transmission. Structural data on GCPII are thus valuable for the design and optimization of GCPII-specific inhibitors and diagnostic probes. The currently available structure of ligand-free GCPII was refined to a resolution of 3.5 Å. This work reports the structure of the protein refined to 1.65 Å resolution, with crystallographic values of R = 0.207 and R_free = 0.228. The new structure extends the resolution appreciably and the new model based on this data shows significant differences when compared with the previously published model.

PDB reference: 2oot

Keywords: prostate-specific membrane antigens; metallopeptidase; folate hydrolases; NAALADase.