Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 62, Part 2 (February 2006)

crystallization communications

Acta Cryst. (2006). F62, 97-99 [ doi:10.1107/S1744309105041011 ]

Crystallization and preliminary crystallographic study of the yeast Malassezia sympodialis allergen Mala s 1

M. Vilhelmsson, B. M. Hallberg, O. Rasool, A. Zargari, A. Scheynius and A. Achour

Abstract: The opportunistic yeast Malassezia sympodialis can act as an allergen and elicit specific IgE- and T-cell reactivity in patients with atopic eczema. The first identified major allergen from M. sympodialis, Mala s 1, is present on the cell surface of the yeast. Recombinant Mala s 1 was expressed in Escherichia coli, purified and refolded in a soluble form. Crystals of Mala s 1 were obtained in 25% PEG 8K, 0.2 M (NH₄)₂SO₄. Crystals belong to space group P2₁2₁2, with unit-cell parameters a = 44.4, b = 163.7, c = 50.6 Å, and diffract to 1.35 Å resolution.

Keywords: Mala s 1; Malassezia sympodialis; recombinant allergen; atopic eczema.