Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 61, Part 10 (October 2005)

crystallization communications

Acta Cryst. (2005). F61, 949-952 [ doi:10.1107/S1744309105029258 ]

Crystallization of the C-terminal domain of the addiction antidote CcdA in complex with its toxin CcdB

L. Buts, N. De Jonge, R. Loris, L. Wyns and M.-H. Dao-Thi

Abstract: CcdA and CcdB are the antidote and toxin of the ccd addiction module of Escherichia coli plasmid F. The CcdA C-terminal domain (CcdA_C36; 36 amino acids) was crystallized in complex with CcdB (dimer of 2 × 101 amino acids) in three different crystal forms, two of which diffract to high resolution. Form II belongs to space group P2₁2₁2₁, with unit-cell parameters a = 37.6, b = 60.5, c = 83.8 Å and diffracts to 1.8 Å resolution. Form III belongs to space group P2₁, with unit-cell parameters a = 41.0, b = 37.9, c = 69.6 Å, [beta] = 96.9°, and diffracts to 1.9 Å resolution.

Keywords: plasmid addiction; toxin-antitoxin systems.