Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 61, Part 10 (October 2005)

crystallization communications

Acta Cryst. (2005). F61, 931-934 [ doi:10.1107/S174430910502854X ]

Crystallization and preliminary X-ray analysis of the Man(1-2)Man-specific lectin from Bowringia mildbraedii in complex with its carbohydrate ligand

A. Garcia-Pino, R. Loris, L. Wyns and L. Buts

Abstract: The lectin from Bowringia mildbraedii seeds crystallizes in the presence of the disaccharide Man( [alpha] 1-2)Man. The best crystals grow at 293 K within four weeks after a pre-incubation at 277 K to induce nucleation. A complete data set was collected to a resolution of 1.90 Å using synchrotron radiation. The crystals belong to space group I222, with unit-cell parameters a = 66.06, b = 86.35, c = 91.76 Å, and contain one lectin monomer in the asymmetric unit.

Keywords: Bowringia mildbraedii; legume lectins; carbohydrate recognition.