Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 61, Part 10 (October 2005)

crystallization communications

Acta Cryst. (2005). F61, 917-921 [ doi:10.1107/S1744309105028514 ]

Production, purification, crystallization and preliminary X-ray structural studies of adeno-associated virus serotype 5

M. DiMattia, L. Govindasamy, H. C. Levy, B. Gurda-Whitaker, A. Kalina, E. Kohlbrenner, J. A. Chiorini, R. McKenna, N. Muzyczka, S. Zolotukhin and M. Agbandje-McKenna

Abstract: Adeno-associated virus serotype 5 (AAV5) is under development for gene-therapy applications for the treatment of cystic fibrosis. To elucidate the structural features of AAV5 that control its enhanced transduction of the apical surface of airway epithelia compared with other AAV serotypes, X-ray crystallographic studies of the viral capsid have been initiated. The production, purification, crystallization and preliminary crystallographic analysis of empty AAV5 viral capsids are reported. The crystals diffract X-rays to beyond 3.2 Å resolution using synchrotron radiation and belong to the orthorhombic space group P2₁2₁2₁, with unit-cell parameters a = 264.7, b = 447.9, c = 629.7 Å. There is one complete T = 1 viral capsid per asymmetric unit. The orientation and position of the viral capsid in the asymmetric unit have been determined by rotation and translation functions, respectively, and the AAV5 structure determination is in progress.

Keywords: adeno-associated virus serotype 5; gene therapy.