Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 61, Part 1 (January 2005)

crystallization communications

Acta Cryst. (2005). F61, 109-111 [ doi:10.1107/S1744309104030829 ]

Crystallization and preliminary X-ray crystallographic study of disproportionating enzyme from potato

K. Imamura, T. Matsuura, Z. Ye, T. Takaha, K. Fujii, M. Kusunoki and Y. Nitta

Abstract: Disproportionating enzyme (D-enzyme; EC 2.4.1.25) is a 59 kDa protein that belongs to the [alpha] -amylase family. D-enzyme catalyses intramolecular and intermolecular transglycosylation reactions of [alpha] -1,4 glucan. A crystal of the D-enzyme from potato was obtained by the hanging-drop vapour-diffusion method. Preliminary X-ray data showed that the crystal diffracts to 2.0 Å resolution and belongs to space group C222₁, with unit-cell parameters a = 69.7, b = 120.3, c = 174.2 Å.

Keywords: disproportionating enzyme; intramolecular and intermolecular transglycosylation reactions.