Acta Crystallographica Section F

Structural Biology and Crystallization Communications

Volume 61, Part 1 (January 2005)

crystallization communications

Acta Cryst. (2005). F61, 87-89 [ doi:10.1107/S1744309104029197 ]

Crystallization and preliminary X-ray diffraction analysis of a lectin from Canavalia maritima seeds

C. A. A. Gadelha, F. B. M. B. Moreno, T. Santi-Gadelha, J. B. Cajazeiras, B. A. M. da Rocha, J. K. R. Rustiguel, B. T. Freitas, F. Canduri, P. Delatorre, W. F. de Azevedo Jr and B. S. Cavada

Abstract: A lectin from Canavalia maritima seeds (ConM) was purified and submitted to crystallization experiments. The best crystals were obtained using the vapour-diffusion method at a constant temperature of 293 K and grew in 7 d. A complete structural data set was collected to 2.1 Å resolution using a synchrotron-radiation source. The ConM crystal belongs to the orthorhombic space group P2₁2₁2, with unit-cell parameters a = 67.15, b = 70.90, c = 97.37 Å. A molecular-replacement search found a solution with a correlation coefficient of 69.2% and an R factor of 42.5%. Crystallographic refinement is under way.

Keywords: lectins; Canavalia maritima.