Journal of Synchrotron Radiation

Volume 13, Part 3 (May 2006)

short communications

J. Synchrotron Rad. (2006). 13, 281-284 [ doi:10.1107/S0909049506004766 ]

High-resolution powder diffraction study of purple membrane with a large Guinier-type camera

T. Oka, K. Miura, K. Inoue, T. Ueki and N. Yagi

Abstract: X-ray diffraction patterns from a film of oriented purple membranes, which comprise two-dimensional crystals of bacteriorhodopsin (BR) trimers, were recorded with a 1 m-pathlength Guinier-type camera at SPring-8 BL40B2. A well focused X-ray beam and a camera with a high angular resolution of 0.024° enabled a powder diffraction profile with very sharp and well separated peaks to be obtained up to a resolution of 2.3 Å. Using integrated diffraction intensities up to a Bragg spacing of 4.2 Å, a cluster of bulky amino acid residues and the head group of the BR chromophore are apparent in the electron density map projected along the membrane normal. Thus, a combination of synchrotron X-rays and large Guinier camera can be used for analyzing the conformational changes of BR in the intact state. In addition, the method might be extended to the structural analysis of film materials composed of two-dimensional arrays of nanoparticles.

Keywords: bacteriorhodopsin; powder diffraction; SPring-8.