Acta Crystallographica Section D

Biological Crystallography

Volume 55, Part 3 (March 1999)

crystallization papers

Acta Cryst. (1999). D55, 669-670 [ doi:10.1107/S0907444998011044 ]

Crystallization and preliminary X-ray analysis of human muscle creatine kinase

L. Tang, H. . Zhou and Z. Lin

Abstract: Creatine kinase is a key enzyme in the energy homeostasis of cells and tissues with high and fluctuating energy demands. Human muscle MM creatine kinase is a dimeric protein with a molecular weight of $\sim$ 43 kDa for each subunit. It has been crystallized by the hanging-drop vapor-diffusion method using 2-methyl-2,4-pentanediol as precipitant. The crystals belong to the enantiomorphous space group or with cell parameters of and Å. The asymmetric unit of the crystal contains two subunits. A data set at 3.3 Å resolution has been collected using synchrotron radiation.

Keywords: creatine kinase; synchrotron radiation.