Acta Crystallographica Section D

Biological Crystallography

Volume 61, Part 4 (April 2005)


research papers



Acta Cryst. (2005). D61, 380-387    [ doi:10.1107/S0907444905000181 ]

Crystallization of mitochondrial respiratory complex II from chicken heart: a membrane-protein complex diffracting to 2.0 Å

L. Huang, T. M. Borders, J. T. Shen, C.-J. Wang and E. A. Berry

Abstract: A procedure is presented for preparation of diffraction-quality crystals of a vertebrate mitochondrial respiratory complex II. The crystals have the potential to diffract to at least 2.0 Å with optimization of post-crystal-growth treatment and cryoprotection. This should allow determination of the structure of this important and medically relevant membrane-protein complex at near-atomic resolution and provide great detail of the mode of binding of substrates and inhibitors at the two substrate-binding sites.

Keywords: succinate dehydrogenase; respiration; membrane proteins; respiratory enzymes; oxidoreductases.

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