Acta Crystallographica Section D

Biological Crystallography

Volume 58, Part 9 (September 2002)

crystallization papers

Acta Cryst. (2002). D58, 1465-1466 [ doi:10.1107/S0907444902010302 ]

Crystallization and preliminary X-ray studies of cold-active protein-tyrosine phosphatase of Shewanella sp.

H. Tsuruta, B. Mikami, C. Yamamoto and Y. Aizono

Abstract: Cold-active protein-tyrosine phosphatase (PTPase) of Shewanella sp. was expressed, purified and crystallized using the hanging-drop vapour-diffusion method at two different pH values (4.6 and 8.5). Both crystals are orthorhombic and belong to space group P2₁2₁2₁, with unit-cell parameters a = 56.4, b = 76.8, c = 81.0 Å (pH 8.5) and a = 57.1, b = 77.0 and c = 81.5 Å (pH 4.6). Diffraction data to 1.82 Å for the pH 8.5 crystal and 2.33 Å for the pH 4.6 crystal were collected on a multiwire area detector using a rotating-anode X-ray generator.

Keywords: protein-tyrosine phosphatase.