Acta Crystallographica Section D

Biological Crystallography

Volume 57, Part 8 (August 2001)

crystallization papers

Acta Cryst. (2001). D57, 1180-1183 [ doi:10.1107/S0907444901009805 ]

Purification, crystallization and identification by X-ray analysis of a prostate kallikrein from horse seminal plasma

A. L. Carvalho, J. M. Dias, L. Sanz, A. Romero, J. J. Calvete and M. J. Romão

Abstract: The purification, crystallization and identification by X-ray diffraction analysis of a horse kallikrein is reported. The protein was purified from horse seminal plasma. Crystals belong to space group C2 and the structure was solved by the MIRAS method, with two heavy-atom derivatives of mercury and platinum. X-ray diffraction data to 1.42 Å resolution were collected at the ESRF synchrotron-radiation source.

Keywords: kallikreins.