Acta Crystallographica Section D

Biological Crystallography

Volume 57, Part 7 (July 2001)

crystallization papers

Acta Cryst. (2001). D57, 1046-1047 [ doi:10.1107/S0907444901007740 ]

Crystallization and preliminary X-ray crystallographic studies on 4--glucanotransferase from Thermotoga maritima

A. Roujeinikova, C. Raasch, S. Sedelnikova, W. Liebl and D. W. Rice

Abstract: Thermotoga maritima 4- [alpha] -glucanotransferase (GTase), a 52 kDa molecular-weight amylolytic enzyme, has been crystallized by the hanging-drop vapour-diffusion method using PEG monomethylether 5000 as a precipitating agent. A complete data set has been collected to 2.6 Å resolution using cryocooling conditions and synchrotron radiation. The crystals belong to space group I222 or I2₁2₁2₁, with unit-cell parameters a = 92.6, b = 180.3, c = 199.2 Å.

Keywords: 4--glucanotransferase; -amylase; Thermotoga maritima.