Acta Crystallographica Section D

Biological Crystallography

Volume 57, Part 7 (July 2001)

crystallization papers

Acta Cryst. (2001). D57, 1030-1031 [ doi:10.1107/S0907444901005571 ]

Preliminary crystallographic study of Thermus aquaticus glycerol kinase

H.-S. Huang, T. Inoue, K. Ito and T. Yoshimoto

Abstract: Glycerol kinase (GlpK) is an important enzyme which catalyzes the rate-limiting step in a central biochemical pathway involving glycerol metabolism. GlpK from the thermophile Thermus aquaticus has been overexpressed in glpK-deficient Escherichia coli and crystallized by the hanging-drop method. The crystal belongs to the cubic space group I23, with unit-cell parameters a = b = c = 163.94 (3) Å. Native data were collected to 2.87 Å resolution on a Cu K [alpha] rotating-anode X-ray source.

Keywords: glycerol kinase.