Acta Crystallographica Section D

Biological Crystallography

Volume 57, Part 5 (May 2001)

crystallization papers

Acta Cryst. (2001). D57, 709-710 [ doi:10.1107/S0907444901002293 ]

Crystallization and preliminary X-ray analysis of cardiotoxic phospholipase A₂ from Ophiophagus hannah (king cobra)

Z. Wang, M. Zhuang, Y. Shu, H. Zhang, S. Song and Z. Lin

Abstract: An acidic phospholipase A₂ exhibiting cardiotoxicity, myotoxicity and anti-platelet activity was isolated from Ophiophagus hannah (king cobra) from Guangxi, China. It contains an unusual `pancreatic loop'. The enzyme was purified to homogeneity and crystallized using polyethylene glycol and ethylene glycol as precipitants. The crystal belongs to space group C2, with unit-cell parameters a = 117.92, b = 62.94, c = 57.16 Å, [beta] = 100.93°. Diffraction data were collected to 2.6 Å.

Keywords: phospholipase A₂; snake venoms.