Acta Crystallographica Section B

Structural Science

Volume 52, Part 3 (June 1996)

research papers

Acta Cryst. (1996). B52, 509-518 [ doi:10.1107/S0108768195014765 ]

Stereochemistry of serotonin receptor ligands from crystallographic data. Crystal structures of NAN-190.HBr, 1-phenylbiguanide, MDL 72222 and mianserin.HCl and selectivity criteria towards 5-HT₁, 5-HT₂ and 5-HT₃ receptor subtypes

A. Dalpiaz, V. Ferretti, P. Gilli and V. Bertolasi

Abstract: The crystal and molecular structures of the following serotoninergic drugs have been determined: (1) 1-(2-methoxyphenyl)-4-[4-(2-phthalimido)butyl]piperazine hydrobromide hemihydrate (NAN-190.HBr), C₂₃H₂₈N₃O₃⁺.Br^-.1/2H₂O, M_r = 483.42, monoclinic, C2/c, a = 21.916 (4), b = 15.207 (2), c = 14.052 (2) Å, = 101.56 (1)°, V = 4588 (1) Å³, Z = 8, D_x = 1.40 Mg m^-3, [lambda] (Mo K [alpha] ) = 0.71069 Å, = 1.823 mm^-1, F(000) = 2008, T = 295 K, R = 0.035 for 2617 observed reflections; (2) N-phenylimidocarbonimidic diamide (1-phenylbiguanide), C₈H₁₁N₅, M_r = 177.21, monoclinic, P2₁/c, a = 9.781 (2), b = 35.040 (5), c = 11.000 (2) Å, = 97.72 (1)°, V = 3736 (1) Å³, Z = 16, D_x = 1.26 Mg m^-3, [lambda] (Mo K [alpha] ) = 0.71069 Å, = 0.084 mm^-1, F(000) = 1504, T = 295 K, R = 0.070 for 3407 observed reflections; (3) 8-methyl-8-azabicyclo[3.2.1.]oct-3-yl 3,5-dichlorobenzoate (MDL 72222), C₁₅H₁₇C₁₂NO₂, M_r = 314.21, triclinic, P ${\bar 1}$ , a = 8.480 (3), b = 9.840 (3), c = 10.15 (4) Å, = 90.04 (3), = 111.77 (3), [gamma] = 105.07 (3)°, V = 755.6 (5) Å³, Z = 2, D_x = 1.38 Mg m^-3, [lambda] (Mo K [alpha] ) = 0.71069 Å, = 0.430 mm^-1, F(000) = 328, T = 295 K, R = 0.070 for 1685 observed reflections; (4) 1,2,3,4,10,14b-hexahydro-2-methyldibenzo[cf]pyrizino[1,2-a]azepine hydrochloride (mianserin.HCl), C₁₈H₂₁N₂⁺.Cl^-, M_r = 300.83, monoclinic, P2₁/a, a = 9.014 (2), b = 14.917 (2), c = 12.412 (2) Å, = 108.84 (1)°, V = 1579.5 (5) Å³, Z = 4, D_x = 1.26 Mg m^-3, [lambda] (Mo K [alpha] ) = 0.71069 Å, = 0.237 mm^-1, F(000) = 640, T = 295 K, R = 0.063 for 1493 observed reflections. A systematic structural analysis of the present compounds and others known to interact with the 5-HT₁, 5-HT₂ and 5-HT₃ receptors allows to identify their similarities with the endogenous ligand serotonin (5-HT) and the stereochemical differences which determine selectivity for the various receptor subtypes. The pharmacophoric feature for 5-HT receptor binding is identified in a constant-length vector linking an aromatic ring with a protonated nitrogen, while specific affinities for receptorial subtypes and the nature of the effect appear to be modulated by the dimensions of the substituents at nitrogen.